Human stem cell bioprocessing

  • HESUB - Collaborative Project FP7 Health - High Efficient Single-Use-Bioreactor simulating 
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 mammalian tissue conditions for expression and proliferation

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HESUB project combines several individual technologies from previous FP projects into one product that will be able to produce enough stem cells for one therapeutic treatment per day per unit. The HESUB product concept is a Single-Use-Bioreactor, which integrates a nanofibre porous scaffold optimised for the proliferation of human stem cells and a sensor package that measures a range of key parameters. The bioreactor aims at providing cost-efficient production of human stem cells for therapeutic treatment. It focuses on myogenic progenitors, as well as human pluripotent stem cells and their differentiation into neural or hepatic fates.

  • Comparison of cultivation in Techne spinner, Bellco spinner, shake flask and T-flask of human embryonic stem cells 

Collaboration: Division of Alzheimer Neurobiology, Clinic of Geriatric Medicine, Karolinska Institute 


The recent progress in regenerative medicine indicates that pluripotent human embryonic stem cells (hESCs) may hold great potential providing cellular models for drug development and screening, modelling diseases as well as aid in the development of future cell-based therapies for neurodegenerative disorders, such as Alzheimer’s and Parkinson’s disease. Crucial to the success of generating specialized cell populations, is an understanding of the mechanisms, which influence the control of cell growth and differentiation by extrinsic and intrinsic factors. Nowadays, a limitation for the use of hESCs is the lack of proliferation methods in large scale. 

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The purpose of the present work is to study several cultivation systems, which could potentially provide 

large-scale cultivation processes suitable for human therapy applications. Pluripotent human embryonic stem cells (hESCs), isolated from the inner cell mass of the blastocyst, are cultivated undifferentiated as embryoids bodies, i.e. large spherical aggregates of cells, in absence of serum and feeder layer. The cell growth and culture behavior in T-flask in hypoxia or normal atmosphere, in Bellco spinner,in Techne spinner and in agitated shake flask are studied.

 

Application of proteomics and transcriptomics to study the proliferation of undifferentiated human embryonic stem cells and establishment of methods for large scale cultivation of these cells. Collaborations: Division of Alzheimer Neurobiology, Clinic of Geriatric Medicine, Karolinska Institute; Protein Array Technologies, KTH/Science for Life Laboratory; Genomics and functional genomics, KTH/Science for Life Laboratory