Comparison of cultivation in Techne spinner, Bellco spinner, shake flask and T-flask of human embryonic stem cells
Collaborations: Division of Alzheimer Neurobiology, Clinic of Geriatric Medicine, Karolinska Institute
The recent progress in regenerative medicine indicates that pluripotent human embryonic stem cells (hESCs) may hold great potential providing cellular models for drug development and screening, modelling diseases as well as aid in the development of future cell-based therapies for neurodegenerative disorders, such as Alzheimer’s and Parkinson’s disease. Crucial to the success of generating specialized cell populations, is an understanding of the mechanisms, which influence the control of cell growth and differentiation by extrinsic and intrinsic factors. Nowadays, a limitation for the use of hESCs is the lack of proliferation methods in large scale.
The purpose of the present work is to study several cultivation systems, which could potentially provide
large-scale cultivation processes suitable for human therapy applications. Pluripotent human embryonic stem cells (hESCs), isolated from the inner cell mass of the blastocyst, are cultivated undifferentiated as embryoids bodies, i.e. large spherical aggregates of cells, in absence of serum and feeder layer. The cell growth and culture behavior in T-flask in hypoxia or normal atmosphere, in Bellco spinner,in Techne spinner and in agitated shake flask are studied.
Application of proteomics and transcriptomics to study the proliferation of undifferentiated human embryonic stem cells and establishment of methods for large scale cultivation of these cells
Collaborations: Division of Alzheimer Neurobiology, Clinic of Geriatric Medicine, Karolinska Institute; Protein Array Technologies, KTH/Science for Life Laboratory; Genomics and functional genomics, KTH/Science for Life Laboratory