Publications

Recent publications

Peer-reviewed publications

  • Ladd, B., Bowes, K., Lundgren, M., Gräslund, T., Chotteau, V., 2022. Proof-of-concept of continuous transfection for adeno-associated virus production in microcarrier-based culture, Processes 2022, 10(3), 515; doi.org/10.3390/pr10030515
  • Scheffel, J., Isaksson, M., Gomis-Fons, J., Schwarz, H., Andersson, N., Norén, B., Solbrand, A., Chotteau, V., Hober, S. and Nilsson, B., 2022. Design of an integrated continuous downstream process for acid-sensitive monoclonal antibodies based on a calcium-dependent Protein A ligand. Journal of Chromatography A, p.462806.
  • Schwarz H, Gomis-Fons J, Isaksson M, Scheffel J, Andersson N, Andersson A, Castan A, Solbrand A, Hober S, Nilsson B, Chotteau V. 2022. Integrated continuous biomanufacturing on pilot scale for acid-sensitive monoclonal antibodies, accepted in Biotechnology and Bioengineering
  • Wang, Y., Pasquini, M., Chotteau, V., Hjalmarsson, H., Jacobsen, E.. Robust optimization with optimal experiment design - with application to continuous biopharmaceutical production", accepted for Proc. DYCOPS 2022
  • Schwarz, H., Mäkinen, M. E., Castan, A., & Chotteau, V. 2022. Monitoring of amino acids and antibody N-glycosylation in high cell density perfusion culture based on Raman spectroscopy. Biochemical Engineering Journal, 182, 108426.
  • Colin K, Hjalmarsson H, Chotteau V. Gaussian process modelling of macroscopic kinetics: a better-tailored kernel for Monod-type kinetics. accepted for Proc. MATHMOD/ IFAC 2022
  • Brechmann, N.A., Jansson, M., Hägg, A., Hicks, R., Hyllner, J., Eriksson, K. and Chotteau, V., 2022. Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads. Magnetochemistry, 8(3), p.34.
  • Mikkonen, S., Josefsson, L., Mäkinen, M. E. L., Chotteau, V., & Emmer, Å. 2022. Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation. Biotechnology Journal, 2100325.
  • Barzadd, M. M., Lundqvist, M., Harris, C., Malm, M., Volk, A. L., Thalén, N., Chotteau, V, ... & Rockberg, J. (2022). Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells. New Biotechnology, 68, 68-76.
  • Wang, Y., Pasquini, M., Chotteau, V., Hjalmarsson, H., Jacobsen, E. 2022. Robust optimization with optimal experiment design - with application to continuous biopharmaceutical production", accepted at DYCOPS 2022
  • Saghaleyni, R., Malm, M., Zrimec, J., Razavi, R., Wistbacka, N., Chotteau, V., ... & Rockberg, J. 2022. Enhanced metabolism and negative regulation of ER stress support higher erythropoietin production in HEK293 cells. Cell Reports accepted 
  • Brechmann, N.A., Jansson, M., Hägg, A., Hicks, R., Hyllner, J., Eriksson, K. and Chotteau, V., 2022. Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads. Magnetochemistry, 8(3), p.34.
  • Mikkonen, S., Josefsson, L., Mäkinen, M.E.L., Chotteau, V., Emmer, Å. 2022. Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation, accepted in Biotechnology Journal
  • Malm, M., Kuo, C.C., Barzadd, M.M., Mebrahtu, A., Wistbacka, N., Razavi, R., Volk, A.L., Lundqvist, M., Kotol, D., Tegel, H., Hober, S., Edfors, F., Gräslund, T., Chotteau, V., Field, R., Varley, P.G., Roth, R.G., Lewis, N.E., Hatton, D., Rockberg, J. 2022. Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins. Metabolic Engineering https://doi.org/10.1016/j.ymben.2022.03.009.
  • Moradi, M., Lundqvist, M., Harris, C., Malm, M., Volk, A.L., Thalén, N., Chotteau, V., Grassi, L., Smith, A., Abadi, M.L. and Lambiase, G., Gibson, S., Hatton, D., Rockberg, J. 2022. Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells. New Biotechnology, 68, pp.68-76.
  • Saghaleyni, R., Malm, M., Zrimec, J., Razavi, R., Wistbacka, N., Chotteau, V., ... & Rockberg, J. Enhanced metabolism and negative regulation of ER stress support higher erythropoietin production in HEK293 cells. Cell Reports 2022 accepted
  • Pinto, I.F., Mikkonen, S., Josefsson, L., Mäkinen, M.E.L., Soares, R.R.G., Russom, A., Emmer, Å., Chotteau, V. 2021. Knowing more from less: miniaturization of ligand-binding assays and electrophoresis as new paradigms for at-line monitoring and control of mammalian cell bioprocesses, accepted in Current Opinion in Biotechnology
  • Särnlund, S., Jiang, Y. and Chotteau, V., 2021. Process intensification to produce a difficult‐to‐express therapeutic enzyme by high cell density perfusion or enhanced fed‐batch. Biotechnology and Bioengineering. doi.org/10.1002/bit.27806
  • Pinto, I.F., Soares, R.R., Mäkinen, M.E.L., Chotteau, V. and Russom, A., 2021. Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production, ACS sensors. 6(3):842-851
  • Brechmann, N.A., Schwarz, H., Eriksson, P.-O., Eriksson, K., Shokri, A. and Chotteau, V. 2021. Antibody capture process based on magnetic beads from very high cell density suspension, Biotechnology and Bioengineering doi.org/10.1002/bit.27776
  • Zhang, L, Wang ML, Castan A, Hjalmarsson H, Chotteau V. 2021. Probabilistic Model by Bayesian Network for the Prediction of Antibody Glycosylation in Perfusion and Fed-Batch Cell Cultures, Biotechnology and Bioengineering doi.org/10.1002/bit.27769
  • Bastin, G., Chotteau, V. and Vande Wouwer, A., 2021. Metabolic Flux Analysis of VERO Cells under Various Culture Conditions. Processes, 9(12), p.2097.
  • van der Burg, D., Josefsson, L., Mikkonen, S., Chotteau, V., Emmer, Å., Wätzig, H. and Sänger‐van de Griend, C.E., 2021. Method development for mono‐and disaccharides monitoring in cell culture medium by capillary and microchip electrophoresis. Electrophoresis. https://doi.org/10.1002/elps.202100213
  • Rodrigues D, Abdalmoaty MR, Jacobsen EW, Chotteau V, Hjalmarsson H. An Integrated Approach for Modeling and Identification of Perfusion Bioreactors via Basis Flux Modes. Computers & Chemical Engineering. 2021 Jan 26:107238.
  • Zhang, L., Schwarz, H., Wang, M., Castan, A., Hjalmarsson, H., Chotteau, V. 2020. Control of IgG glycosylation in CHO cell perfusion cultures by GReBA mathematical model supported by a novel targeted feed, TAFE. Metabolic Engineering 65:135-145 https://doi.org/10.1016/j.ymben.2020.11.004.
  • Malm, M., Saghaleyni, R., Lundqvist, M., Giudici, M., Chotteau, V., Field, R., Varley, P., G., Hatton, D., Grassi, L., Svensson, T., Nielsen, J. and Johan Rockberg2020. Evolution from adherent to suspension – systems biology of HEK293 cell line development. Scientific Reports 10, 18996 https://doi.org/ 10.1038/s41598-020-76137-8
  • Wang, M., Jacobsen, E., Chotteau, V., Hjalmarsson, H. 2020. Estimation of Heteroscedastic Multilinear Systems. Proc. 59th IEEE Conference on Decision and Control (CDC). regular paper
  • Zhan, C., Bidkhori, G., Schwarz, H., Malm, M., Mebrahtu, A., Field, R., ... & Chotteau, V. 2020. Low shear stress increases recombinant protein production and high shear stress increases apoptosis in human cells. iScience, 101653 DOI:https://doi.org/10.1016/j.isci.2020.101653
  • Åstrand C., Chotteau V., Falk A., Hedhammar M. 2020. Assembly of FN-silk with laminin-521 to integrate hPSCs into a three-dimensional culture for neural differentiation. Biomaterials Science. 8(9):2514-25
  • Gomis-Fons, J., Schwarz, H., Zhang, L., Andersson, N., Nilsson, B., Castan, A., Solbrand, A., Stevenson, J., Chotteau, V. 2020 Model-based design and control of a small-scale integrated continuous end-to-end mAb platform. Biotechnology Progress e2995
  • Wang, M., Risuleo, R., S., Jacobsen, E., W., Chotteau, V., Hjalmarsson, H. 2020. Identification of Nonlinear Kinetics of Macroscopic Bio-reactions Using Multilinear Gaussian Processes. Computers and Chemical Engineering 133: 106671
  • Zhang, L., Wang, M., Castan, A., Stevenson, J., Chatzissavidou, N., Hjalmarsson, H., Vilaplana, F., Chotteau, V. 2020. Glycan Residues Balance Analysis-GReBA: A novel model for the N-linked glycosylation of IgG produced by CHO cells. Metabolic engineering 57: 118-128
  • Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. 2019. Small-scale bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin. PMID: 31891733. Journal of Biotechnology 309:44-52. doi: 10.1016/j.jbiotec.2019.12.017
  • Hagrot, E, Hildur Oddsdóttir Æ, Forsgren A, Chotteau V. 2019. Novel column generation-based optimization approach for large poly-pathway kinetic model applied to CHO cell culture. Metabolic Engineering Communications 8: e00083
  • Zhang, L , Castan , A, Stevenson , J, Chatzissavidou , N, Vilaplana , F, Chotteau , V. 2019. Combined Effects of Glycosylation Precursors and Lactate on the Glycoprofile of IgG Produced by CHO Cells. Journal of Biotechnology 289, 71-79 https://doi.org/10.1016/j.jbiotec.2018.11.004
  • Brechmann, N.A., Eriksson, P.-O., Eriksson, K., Oscarsson, S., Buijs, J., Shokri, A., Hjälm, G. and Chotteau, V. 2019. Pilot‐scale process for magnetic bead purification of antibodies directly from non‐clarified CHO cell culture. Biotechnology progress 2019-Jan 10:e2775
  • Wang, M., Jacobsen, E. W., Chotteau, V., & Hjalmarsson, H. 2019. A Multi-Step Least-Squares Method for Nonlinear Rational Models. Proc. IEEE. American Control Conference (ACC), Philadelphia, PA, USA, 2019, July, pp. 4509-4514
  • Zhan, C., Hagrot, E., Brandt, L., Chotteau, V. 2019. Study of hydrodynamics in Wave Bioreactors by Computational Fluid Dynamics reveals a resonance phenomenon, Chemical Engineering Science 193:53-65. doi.org/10.1016/j.ces.2018.08.017
  • Zamani, L., Lundqvist, M., Zhang, Y., Aberg, M., Edfors, F., Bidkhori, G., Lindahl, A., Mie, A., Mardinoglu, A., Field, R., Turner, R., Rockberg, J., Chotteau, V. 2018. High cell density perfusion culture has a maintained exoproteome and metabolome, Biotechnology Journal, doi.org/10.1002/biot.201800036
  • Leino, M., Åstrand, C., Hughes-Brittain, N., Robb, B., McKean, R., Chotteau, V. 2018. Human embryonic stem cell dispersion in electrospun PCL fibre scaffolds by coating with laminin-521 and E-cadherin-Fc, Journal of Biomedical Materials Research: Part B - Applied Biomaterials J Biomed Mater Res Part B: Appl Biomater, 106B: 1226–1236, DOI: 10.1002/jbm.b.33928
  • Ravichandra, R., Åstrand, C., Patra, H. K., Turner, A. P. F., Chotteau, V., Phopase, J. 2017. Intelligent ECM-mimetic Injectable Scaffolds Based on Functional Collagen Building Blocks for Tissue Engineering and Biomedical applications, RSC Advances 7:21068-21078
  • Hagrot, E., Hildur Oddsdóttir, Æ., Gonzalez Hosta, J., Jacobsen, E.W., Chotteau, V. 2017. Poly-pathway model, a novel approach to simulate multiple metabolic states by reaction network-based model - Application to CHO cell culture', 2017, Journal of Biotechnology 259:235-247
  • Al-Khalili, L., Gillner, K., Zhang, Y., Åstrand, C., Shokri, A., Hughes-Brittain, N., McKean, R., Robb, B., Chotteau, V. 2016. Characterization of human CD133+ cells in biocompatible poly-L-lactic acid electrospun nano-fiber scaffolds. Journal of Biomaterials and Tissue Engineering 6:959-966
  • Zhang, Y., Stobbe, P., Orrego, C.S., Chotteau, V. 2015. Very high cell density perfusion of CHO cells anchored in a non-woven matrix-based bioreactor. Journal of Biotechnology 213:28-41
  • Zhang, Y., Chotteau., V. 2015. Observation of Chinese Hamster Ovary Cells retained inside the non-woven fiber matrix of the CellTank bioreactor. Data in Brief 5:586-588
  • Hildur Oddsdóttir, Æ., Hagrot, E., Chotteau, V., Forsgren, A. 2015. Robust generation of elementary flux modes. Mathematical Biosciences 273:45-56
  • Hildur Oddsdóttir, Æ., Hagrot, E., Chotteau, V., Forsgren, A. 2015. On Dynamically Generating Relevant Elementary Flux Modes in a Metabolic Network using Optimization. Journal of Mathematical Biology 71:903-920
  • Clincke, M.-F., Mölleryd, C., Zhang, Y., Lindskog, E., Walsh, K., Chotteau, V. 2013. Very High Density of CHO Cells in Perfusion by ATF or TFF in WAVE Bioreactor™ - Part I. Effect of the Cell Density on the Process. Biotechnology Progress, 29:754-767
  • Clincke, M.-F., Mölleryd, C., Samani, P.K., Lindskog, E., Fäldt, E., Walsh, K., Chotteau, V. 2013 Very High Density of CHO Cells in Perfusion by ATF or TFF in WAVE Bioreactor™ – Part II: Applications for Antibody Production and Cryopreservation. Biotechnology Progress, 29:768-777


Book chapters

  • Chotteau, V., Hagrot, E., Zhang, L. and Mäkinen, M.E., 2021. ‘Mathematical Modelling of Cell Culture Processes’. book chapter in Cell Culture Engineering and Technology (pp. 431-466). Springer, Cham.
  • Chotteau V, 'Perfusion Processes', book chapter in Animal Cell Culture, ed Al-Rubeai M, Springer Intl., Volume 9, 2015, pp 407-443
  • Chotteau V, Zhang Y, Clincke M-F. 2014. 'Very high cell density in perfusion of CHO cells by ATF, TFF, Wave bioreactor and/or CellTank technologies - Impact of cell density and applications', book chapter in Continuous Processing in Pharmaceutical Manufacturing, ed Subramanian, S, Wiley, pp 339-356